Whole genome sequencing (WGS) refers to the comprehensive examination of a genome by reading and stitching together short fragments to determine an organism’s complete chromosomal (nuclear) and mitochondrial DNA sequence.
Exome is a term used to describe the sum of all regions in the genome comprised of exons. Exons are DNA regions that are transcribed into messenger RNA, as opposed to introns which are removed by splicing proteins.
Metagenomics, the field can be defined as the genomic analysis of microbial DNA from environmental communities. Metagenomics tools enable the population analysis of un-culturable or previously unknown microbes.
RNA-Seq offers a snapshot of the entire transcriptome or messenger RNA (mRNA) profile at a given moment in time. RNA-Seq allows for the detection of transcript isoforms, allele specific gene expression, gene fusions, and single nucleotide variants.
Genotyping by sequencing is a genetic screening method for discovering novel plant and animal SNPs. Sequence-based genotyping provides a lower-cost alternative to arrays for studying genetic variation.
Single-cell sequencing examines the genomes or transcriptomes of individual cells, providing a high-resolution view of cell-to-cell variation. These assays enhance the study of cell function and heterogeneity in time-dependent processes such as differentiation, proliferation, and tumorigenesis.
